PES Membrane Filter, 300 kDa NMWCO. View Price and Availability. Z629480. Vivaspin 20 centrifugal concentrator. View Price and Availability. Z629499. ... Plant; Application. These 0.5 mL centrifugal filter enables the concentration of biomolecules with a NMWL of 300kDa.
WhatsApp: +86 18221755073Navbar Search Filter Mobile Enter search term Search. Close. Navbar Search Filter ... Although lower amounts of CysP13 (RM2 and RM4) prolamins were also observed by Kawakatsu et al. (2010), their 10 kDa prolamin-suppressed plant accumulated substantially higher levels (about three times compared with the wild type) of CysR14 (RM1 and RM9) and ...
WhatsApp: +86 18221755073The UF process with the 3 kDa membrane produced a protein concentration in the retentate between 55% and 70%, while reducing stachyose content from 6% to 2%. The …
WhatsApp: +86 18221755073For both lines, wild-type (WT) plants (TIC12/TIC12) and heterozygous plants (tic12-1/TIC12 and tic12-2/TIC12) showed normal growth indistinguishable from the WT . In sharp contrast, homozygous tic12-1 and tic12-2 mutants showed severe albino, seedling-lethal phenotypes just like the tic20-I null mutant.
WhatsApp: +86 18221755073SDS-PAGE analysis of triple-cycled microtubule protein revealed the presence of 120 kDa, 110 kDa and a group of 60-65 kDa polypeptides in addition to tubulin. Since these polypeptides had copolymerized with tubulin, through the three cycles of assembly and disassembly, and they bundle microtubules, we tentatively identified the three ...
WhatsApp: +86 18221755073We have shown that the 200 kDa protein is the largest member of a family of immunologically-related cold-induced proteins in wheat. Expression of pWcs200 in E. coli yielded a product of around 200 kDa, indicating that the clone contains most …
WhatsApp: +86 18221755073CRUMPLED LEAF interacts with OUTER ENVELOPE PROTEIN OF 80 KDA to regulate its complex formation in the outer envelope membrane of Arabidopsis chloroplasts. ...
WhatsApp: +86 18221755073The isolated P-115-ABP type of plant villin was widely distributed in plant cells, from algae to land plants and showed co-localization with actin-filament cables anchored on the intracellular surface of chloroplasts, indicating that plant villins are widely distributed and involved in the organization of actin filaments into bundles throughout the plant kingdom. In many …
WhatsApp: +86 18221755073FOS can be extracted directly from natural plants. For example, yacon roots are considered an alternative source due to their high FOS contents. ... (84.15 %) and xylan (87.45 %). Singh et al. [56] discovered that a 10 kDa ultrafiltration membrane effectively eliminates high molecular-weight components. At the same time, a 150 Da nanofiltration ...
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WhatsApp: +86 18221755073PES Membrane Filter, 300 kDa NMWCO. View Price and Availability. Millipore. PBMK04710. PES Membrane Filter, 300 kDa NMWCO. View Price and Availability. Sigma-Aldrich. 682659. ... Plant; Application. These 0.5 mL centrifugal filter enables the concentration of biomolecules with a NMWL of 300kDa.
WhatsApp: +86 18221755073The 13-kDa group was found to be composed of two polypeptides of slightly different molecular sizes, 13a (larger component) and 13b (smaller component). Most of the 13a and 13b polypeptides were shown to be largely prolamins, although there were also some salt- and alcohol-insoluble polypeptides with an apparent molecular size of 13 kDa.
WhatsApp: +86 18221755073The protein migrated as a single band on sodium dodecyl sulfate-polyacrylamide gels with an estimated molecular mass of 22.5 kDa. The exact mass was determined by mass …
WhatsApp: +86 18221755073Amicon ® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95%. The vertical design and available membrane surface area provide fast sample …
WhatsApp: +86 18221755073Photos of the experimental setup: (A) vetiver plant in beaker, (B) vetiver plant inside filter case, (C) vetiver plant + filter case setup, and (D) schematic filter case setup. Additionally, the heavy metal uptake within the roots ( Fig. 6 ) and shoots ( Fig. 7 ) of the vetiver plants with and without C422-filter show major differences.
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WhatsApp: +86 18221755073Abstract. Pea plants were grown under intermittent illumination (ImL) conditions. The low dosage of light given to ImL plastids limited the rate of chlorophyll (Chl) a and Chl b biosynthesis and, therefore, it retarded the rate of photosynthetic unit formation and thylakoid membrane development. Depending on the developmental stage of the photosynthetic unit, …
WhatsApp: +86 18221755073Amicon ® Ultra 4 mL centrifugal devices with Ultracel ® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95% . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically …
WhatsApp: +86 18221755073In addition, we found that the 16.9-kDa HSP obtained by cleavage of the recombinant protein could also form a protein complex of ˜310 kDa under non-denaturing conditions as can the small, native, class I HSPs from heat-shocked rice seedlings.
WhatsApp: +86 18221755073Membrane filter: 10 kDa MWCO: Cell culture [106] Hollow fiber membrane: 100 kDa MWCO: Cell culture [107 ... copyright 2016 Royal Society of Chemistry (Creative …
WhatsApp: +86 18221755073SDS-PAGE analysis showed that isolated PB-I contains several polypeptide groups, the largest having an apparent molecular size of 13 kDa and two smaller ones of 10 kDa and 16 kDa. The 13-kDa group was found to be composed of two polypeptides of slightly different molecular sizes, 13a (larger component) and 13b (smaller component).
WhatsApp: +86 18221755073Precursor-accumulating (PAC) vesicles were previously shown to mediate the transport of the precursor of a major storage protein (pro2S albumin) to protein-storage vacu-oles in developing pumpkin cotyledons. In this study, we characterized two homologous proteins from PAC vesicles, a 72 kDa protein (PV72) and an 82 kDa protein (PV82).
WhatsApp: +86 18221755073Abstract. We isolated a cDNA for a gene that encodes the 23 kDa protein of the oxygen-evolving complex of photosystem II in rice. The amino acid sequence deduced from the cDNA is highly similar (71–60% homology) to those of the analogous genes from wheat, tomato, pea, spinach, tobacco and mustard.
WhatsApp: +86 18221755073Abstract. Changes in protein kinase activities in Dunaliella tertiolecta in response to hypo- and hyperosmotic shocks were assessed by assays of protein kinase activities on SDS-polyacrylamide gels that contained protein substrates, Hypoosmotic shock (transfer from 0.5 to 0.2 M Nacl) transiently activated a 40-kDa protein kinase (low osmotic pressure-activated …
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WhatsApp: +86 18221755073Navbar Search Filter Mobile Enter search term Search. Issues More Content Advance Articles Editor's Choice ... A New 4.8-kDa Polypeptide Intrinsic to the PS II Reaction Center, as Revealed by Modified SDS-PAGE with Improved Resolution of Low-Molecular-Weight Proteins ... The Japanese Society of Plant Physiologists. Issue Section: Articles ...
WhatsApp: +86 18221755073Amicon ® Ultra Centrifugal Filter, 100 kDa MWCO. View Price and Availability. Millipore. PBMK15005. PES Membrane Filter, 300 kDa NMWCO. ... Plant; Application. These 0.5 mL centrifugal filter enables the concentration of biomolecules with a NMWL of 300kDa.
WhatsApp: +86 18221755073The increased freezing tolerance of ABA-treated cells was closely associated with the remarkable accumulation of 19-kDa polypeptides in the plasma membrane. The 19-kDa polypeptide components were isolated by preparative gel electrophoresis and were further separated into one major (AWPM-19) and other minor polypeptide components by Tricine-SDS ...
WhatsApp: +86 18221755073Abstract. The extrinsic 12 kDa protein in red algal photosystem II (PSII) functions to minimize the chloride and calcium requirement of oxygen-evolving activity [Enami et al. (1998)Biochemistry 37: 2787].In order to identify functional domains of the 12 kDa protein, we prepared the 12 kDa protein lacking N-terminal peptides or C-terminal peptides or both by …
WhatsApp: +86 18221755073The higher plant 33 kDa protein was released and purified from rice and spinach PSII membranes with the method described in Enami et al. (2000). All of the 33 kDa proteins were dialyzed against 50 mM MES-NaOH (pH 6.5) and stored at –80°C, before being subjected to limited proteolysis. Limited proteolysis
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